Mitochondrial RNA Transcript Analysis Assay of Arabidopsis Leaf Tissues
Etienne Delannoy,
Andéol Falcon de Longevialle,
Catherine Francs-Small
Affiliations
Etienne Delannoy
ARC Centre of excellence in Plant Energy Biology, The University of Western Australia, Crawley, AustraliaParis-Saclay Institute of Plant Sciences (IPS2), UMR 1403 INRA-CNRS-Paris 11-Paris 7-UEVE, Evry cedex, France
Andéol Falcon de Longevialle
ARC Centre of excellence in Plant Energy Biology, The University of Western Australia, Crawley, AustraliaLaboratoire Genopole Plant Process Innovation, Bâtiment G1, EVRY cedex, France
Catherine Francs-Small
ARC Centre of excellence in Plant Energy Biology, The University of Western Australia, Crawley, Australia
This qPCR-based assay provides an overview of the expression levels of all mitochondrial transcripts (mRNAs and rRNAs) as well as splicing efficiency in Arabidopsis. It was developed before RNAseq techniques were widely used (de Longevialle et al., 2007), but is nevertheless still useful as it is cheaper to run and the analysis is much easier and faster to perform if the aim is only to look at mitochondrial transcripts. For intron-containing mRNAs, the use of primer sets specifically amplifying spliced or unspliced forms allows the evaluation of the splicing efficiency.
