Effects of age, sex, medication, and environmental conditions on genetic alterations in oral mucosa cells
Deborah Navit de Carvalho Cavalcante,
Bruno do Amaral Crispim,
Beatriz Barufatti Grisolia,
Lucilene Finoto Viana,
Nayara Halimy Maran,
Julio César Jut Solórzano,
Kelly Mari Pires de Oliveira,
Alexeia Barufatti
Affiliations
Deborah Navit de Carvalho Cavalcante
Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil
Bruno do Amaral Crispim
Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil
Beatriz Barufatti Grisolia
Universidade Estadual de Maringá, Av. Colombo, 5790 – Jd. Universitário, CEP 87020-900, Maringá, PR, Brazil
Lucilene Finoto Viana
Universidade Estadual do Mato Grosso do Sul, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 351, CEP 79804-970, Dourados, MS, Brazil
Nayara Halimy Maran
Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil
Julio César Jut Solórzano
Universidade Estadual do Mato Grosso do Sul, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 351, CEP 79804-970, Dourados, MS, Brazil
Kelly Mari Pires de Oliveira
Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil
Alexeia Barufatti
Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 364, CEP 79804-970, Dourados, MS, Brazil; Corresponding author.
In the present study, we evaluated the effects of biological factors, lifestyle factors, and environmental conditions on the induction of DNA damage in exfoliated cells of the oral mucosa. Age, sex, medication use, and environmental conditions were analyzed in individuals residing in the cities of Caarapó and Itaporã. The individuals were assessed by a questionnaire, and oral mucosa cells were collected and subjected to mutagenicity analysis. We observed no statistical differences in DNA damage related to sex. However, the mutagenic effect was found to be proportional to age, with higher frequencies of DNA damage observed in individuals between the ages of 46 and 65 years. In addition, higher frequencies of DNA damage were found in individuals who continuously used medication and for prolonged periods, and greater DNA damage was observed in individuals who used antihypertensive drugs than those who took antidepressants. In terms of environmental conditions, Caarapó residents had a significantly higher frequency of DNA damage than that of residents from Itaporã. Based on the analysis of land use and occupation, this result can be attributed to the smaller fraction of forest fragments and the higher proportion of buildings in Caarapó than Itaporã. We concluded that age, continued medication use, and environmental conditions can lead to greater DNA damage.
