Morphology and Multi-Gene Phylogeny Reveal a New Species of Family Torulaceae from Yunnan Province, China
Shucheng He,
Deping Wei,
Chitrabhanu S. Bhunjun,
Ruvishika S. Jayawardena,
Vinodhini Thiyagaraja,
Qi Zhao,
Al-Otibi Fatimah,
Kevin D. Hyde
Affiliations
Shucheng He
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Deping Wei
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China
Chitrabhanu S. Bhunjun
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
Ruvishika S. Jayawardena
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
Vinodhini Thiyagaraja
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Qi Zhao
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Al-Otibi Fatimah
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia
Kevin D. Hyde
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
The Family Torulaceae belongs to the Order Pleosporales (Class Dothideomycetes) and mainly comprises saprobes. The taxa are widely distributed in both terrestrial and aquatic habitats. In this study, we collected three dead leaf specimens of Carex baccans and two submerged wood specimens in Yunnan Province, China. A biphasic approach of morphological examination and multi-locus phylogenetic analyses conducted for internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), nuclear large subunit rDNA (28S), nuclear small subunit rDNA (18S), translation elongation factor 1-α (tef1) gene, and RNA polymerase II second-largest subunit (rpb2) revealed one new species Rutola kunmingensis and a new collection of Torula sundara. Rutola kunmingensis is characterized by black, powdery colonies, micronematous, creeping, reticular conidiophores bearing inconspicuous, monoblastic conidiogenous loci, and multi-septate, catenulate, verruculose, brown conidia. The conidiophores and conidia of each genus in Torulaceae are mapped onto the phylogenetic tree and the generic demarcations of this family are discussed and the significant divergence of ITS, 18S, 28S, rpb2, and tef1 sequences in Torulaceae is also discussed.
