FMD virus type Asia-1 irradiated vaccine and evaluation of immune response on guinea-pig model

Abstract

Introduction: Foot and Mouth Disease (FMD) is the most contagious disease in cloven-hoofed animals which causes a lot of economical losses. FMD virus belongs to Picornaviridae family and Aphthovirus genus. The aim of the study is evaluation of humoral and cellular immune responses triggered by a Gamma-irradiated FMD vaccine in the guinea-pig model. Materials and Methods: FMD virus type Asia-1 was multiplied on BHK21 cell line and irradiated by gamma ray in different doses. According to dose/survival curve, D10 value and optimum dose of virus inactivation were calculated 7.69 and 50 kGy, respectively. Antigenic characteristic of irradiated and un-irradiated virus samples were evaluated by complement fixation test (CF test) and safety test was done by four blind passages cell culture on IBRS2 cell line. The inactivated virus sample was formulated as Radio- vaccine and immune responses were evaluated in three groups of ginea-pigs; the first group Radio-vaccine, the second group conventional vaccine and the last one was negative control. Results: The results of neutralizing antibody titration for two vaccinated groups were significant (P

Keywords

• FMD virus
• Gamma irradiation
• Vaccine
• Immune Response