Autoimmunity (Oct 2020)

Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes

  • Mohammad Sajid,
  • Krishna Biswas,
  • Harpreet Singh,
  • Sapna Negi

DOI
https://doi.org/10.1080/08916934.2020.1818232
Journal volume & issue
Vol. 53, no. 7
pp. 385 – 393

Abstract

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The depletion of gut mucosal barrier enables exposure of gut microbes/gut microbial products to the host mucosal immunity which may increase the risk of metabolic/inflammatory disorders. These immune responses can lead to the development of mild autoimmunity to metabolic peptides coming from gut bacteria and may result in metabolic diseases like late-onset diabetes (LOD). In the present study, we identified host sera cross-reactivity with gut bacterial peptides similar to host proteins. The interaction between diabetic sera and gut peptides was detected by enzyme-linked immunosorbent assay (ELISA) and results were confirmed using surface plasmon resonance (SPR). The ELISA assay showed a higher level of serum cross-reactivity in LOD patients as compared to non-diabetic controls against three peptides (P-5, P-9, and P-13). SPR analysis confirmed binding-affinity against P-5 and P-13. Also, a significant correlation was observed between inflammatory markers and P-5. This study demonstrates that gut health is important not only for intestinal diseases but also for several late-onset diseases, like, diabetes.

Keywords