Frontiers in Cell and Developmental Biology (Apr 2022)
Effects of the Zbtb1 Gene on Chromatin Spatial Structure and Lymphatic Development: Combined Analysis of Hi-C, ATAC-Seq and RNA-Seq
- Junhong Wang,
- Junhong Wang,
- Junhong Wang,
- Junhong Wang,
- Chunwei Shi,
- Chunwei Shi,
- Chunwei Shi,
- Chunwei Shi,
- Mingyang Cheng,
- Mingyang Cheng,
- Mingyang Cheng,
- Mingyang Cheng,
- Yiyuan Lu,
- Yiyuan Lu,
- Yiyuan Lu,
- Yiyuan Lu,
- Xiaoyu Zhang,
- Xiaoyu Zhang,
- Xiaoyu Zhang,
- Xiaoyu Zhang,
- Fengdi Li,
- Fengdi Li,
- Fengdi Li,
- Fengdi Li,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Xiaoxu Li,
- Xiaoxu Li,
- Xiaoxu Li,
- Xiaoxu Li,
- Xinyang Li,
- Xinyang Li,
- Xinyang Li,
- Xinyang Li,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Chunfeng Wang,
- Chunfeng Wang,
- Chunfeng Wang,
- Chunfeng Wang,
- Xin Cao,
- Xin Cao,
- Xin Cao,
- Xin Cao
Affiliations
- Junhong Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Junhong Wang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Junhong Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Junhong Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chunwei Shi
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chunwei Shi
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chunwei Shi
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chunwei Shi
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Mingyang Cheng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Mingyang Cheng
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Mingyang Cheng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Mingyang Cheng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yiyuan Lu
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yiyuan Lu
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yiyuan Lu
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yiyuan Lu
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xiaoyu Zhang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xiaoyu Zhang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Xiaoyu Zhang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xiaoyu Zhang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Fengdi Li
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Fengdi Li
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Fengdi Li
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Fengdi Li
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yu Sun
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yu Sun
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yu Sun
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yu Sun
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xiaoxu Li
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xiaoxu Li
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Xiaoxu Li
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xiaoxu Li
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xinyang Li
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xinyang Li
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Xinyang Li
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xinyang Li
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan Zeng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chunfeng Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chunfeng Wang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chunfeng Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chunfeng Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Cao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xin Cao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fcell.2022.874525
- Journal volume & issue
-
Vol. 10
Abstract
Zbtb1 (zinc finger and BTB domain containing 1) is a member of mammalian zbtb gene family. A series of bioinformatics analysis was carried out for the EL4 cell and the Zbtb1-deficient EL4 cell by Hi-C, ATAC-seq and RNA-seq techniques. Finally, Hi-C results showed that the intensity of chromatin interaction in the deletion group decreased with distance, the degree of chromosome interaction decreased significantly, the AB division region changed significantly, and the compactness of TAD structure decreased; The results of ATAC-seq showed that the open area and degree of chromatin in the deletion group decreased; 7778 differentially expressed mRNAs were found by RNA-seq. Our experimental results for the first time expounded the significance of Zbtb1 gene for T cell development, lymphocyte production and apoptosis from the aspects of chromosome spatial structure and chromatin opening degree, and provided relevant theoretical basis and data support for the in-depth study of related Zbtb1 genes in the future.
Keywords
