Dose-dependent effects of anthocyanin supplementation on platelet function in subjects with dyslipidemia: A randomized clinical trial
Zezhong Tian,
Kongyao Li,
Die Fan,
Yimin Zhao,
Xiaoli Gao,
Xilin Ma,
Lin Xu,
Yilin Shi,
Fuli Ya,
Jinchao Zou,
Ping Wang,
Yuheng Mao,
Wenhua Ling,
Yan Yang
Affiliations
Zezhong Tian
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Kongyao Li
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Die Fan
Clinical Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong Province 518107, PR China
Yimin Zhao
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Xiaoli Gao
The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong Province 518033, PR China
Xilin Ma
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Lin Xu
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Yilin Shi
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China; Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, PR China
Fuli Ya
Institute of Preventive Medicine, School of Public Health, Dali University, Dali, Yunnan 671000, PR China
Jinchao Zou
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Ping Wang
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Yuheng Mao
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China
Wenhua Ling
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China; Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, PR China
Yan Yang
School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, PR China; Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou, Guangdong Province 510080, PR China; Corresponding author at: School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong Province 518106, PR China.
Background: Dyslipidemia induces platelet hyperactivation and hyper-aggregation, which are linked to thrombosis. Anthocyanins could inhibit platelet function in vitro and in mice fed high-fat diets with their effects on platelet function in subjects with dyslipidemia remained unknown. This study aimed to investigate the effects of different doses of anthocyanins on platelet function in individuals with dyslipidemia. Methods: A double-blind, randomized, controlled trial was conducted. Ninety-three individuals who were initially diagnosed with dyslipidemia were randomly assigned to placebo or 40, 80, 160 or 320 mg/day anthocyanin groups. The supplementations were anthocyanin capsules (Medox, Norway). Platelet aggregation by light aggregometry of platelet-rich plasma, P-selectin, activated GPⅡbⅢa, reactive oxygen species (ROS), and mitochondrial membrane potential were tested at baseline, 6 weeks and 12 weeks. Findings: Compared to placebo group, anthocyanins at 80 mg/day for 12 weeks reduced collagen-induced platelet aggregation (-3.39±2.36%) and activated GPⅡbⅢa (-8.25±2.45%) (P < 0.05). Moreover, compared to placebo group, anthocyanins at 320 mg/day inhibited collagen-induced platelet aggregation (-7.05±2.38%), ADP-induced platelet aggregation (-7.14±2.00%), platelet ROS levels (-14.55±1.86%), and mitochondrial membrane potential (7.40±1.56%) (P < 0.05). There were dose-response relationships between anthocyanins and the attenuation of platelet aggregation, mitochondrial membrane potential and ROS levels (P for trend <0.05). Furthermore, significantly positive correlations were observed between changes in collagen-induced (r = 0.473) or ADP-induced (r = 0.551) platelet aggregation and ROS levels in subjects with dyslipidemia after the 12-week intervention (P < 0.05). Interpretation: Anthocyanin supplementation dose-dependently attenuates platelet function, and 12-week supplementation with 80 mg/day or more of anthocyanins can reduce platelet function in individuals with dyslipidemia.
