Current Research in Physiology (Jan 2021)
Aqueous extract of Tetrapleura tetraptera fruit peels influence copulatory behavior and maintain testicular integrity in sexually mature male Sprague-Dawley rats: Pro-fertility evaluation and histomorphometry evidence
Abstract
Tetrapleura tetraptera (TT) has been used as a spice, dietary supplement and medicine for various ailments. This study evaluate influence of Tetrapleura tetraptera extract on testis and copulatory behavior in sexually mature male rats.Thirty-two male and sixty-four virgin female rats weighing 150–200 g were used for this study. Male rats randomly divided into four groups of eight (n = 8) rats each. Group A: Control given 2 ml distilled water, group B, C and D received 50, 300 and 700 mg/kg bwt TT for 56 days through oral gavage. The female rats were used for fertility test. Testicular histology, histomorphology, copulatory behavior, sperm parameters, testosterone (TET), luteinizing hormone (LH), follicle stimulating hormone (FSH), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and fertility test were investigated.Tetrapleura tetraptera significantly increase sperm count, motility, normal morphology, daily sperm production, efficiency of sperm production, sperm (average path velocity, straight line velocity and curvilinear velocity), TET, LH, FHS, SOD, GPx, CAT, number of pregnant females, number of fetuses, seminiferous diameter, epithelium thickness and decrease abnormal morphology, seminiferous height, tubule lumen and MDA across the group as compared with control group. Improved testicular histological integrity, sexual behaviour and libido by increased frequencies of mount, intromission, ejaculation and ejaculatory latency. Latencies of mount, intromission and post-ejaculation were significantly reduced. Also, observed increase spermatocytes and spermatids showed no significant difference in spermatogonia cell counts. Tetrapleura tetraptera therefore, enhance steroidogenesis, spermatogenesis, and improved testicular histological integrity and boost sexual competence in male rats.
