Communications Biology (May 2021)

A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

  • Pascal Püllmann,
  • Anja Knorrscheidt,
  • Judith Münch,
  • Paul R. Palme,
  • Wolfgang Hoehenwarter,
  • Sylvestre Marillonnet,
  • Miguel Alcalde,
  • Bernhard Westermann,
  • Martin J. Weissenborn

DOI
https://doi.org/10.1038/s42003-021-02076-3
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 20

Abstract

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Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for other genes of interest that are suitable for production in yeast.