pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
S. Boukthir,
P. Gaudu,
A. Faili,
S. Kayal
Affiliations
S. Boukthir
CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France; Inserm, CIC 1414, Rennes, France; Corresponding author. CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.
P. Gaudu
Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
A. Faili
Inserm, CIC 1414, Rennes, France; OSS- Oncogenesis, Stress, Signaling, INSERM 1242, Rennes, France; Université de Rennes, Faculté de Pharmacie, Rennes, France
S. Kayal
CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France; Inserm, CIC 1414, Rennes, France; OSS- Oncogenesis, Stress, Signaling, INSERM 1242, Rennes, France; Université de Rennes, Faculté de Médecine, Rennes, France; Corresponding author. CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.
