Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue

Abstract

The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres.

Keywords

• saffron
• collagen fibres
• multichromatic staining
• connective tissue