Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress
Ting-Ting Yang,
Jia-Jia Qi,
Bo-Xing Sun,
He-Xuan Qu,
Hua-Kai Wei,
Hao Sun,
Hao Jiang,
Jia-Bao Zhang,
Shuang Liang
Affiliations
Ting-Ting Yang
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Jia-Jia Qi
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Bo-Xing Sun
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
He-Xuan Qu
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Hua-Kai Wei
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Hao Sun
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Hao Jiang
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Jia-Bao Zhang
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
Shuang Liang
Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China
As a pentacyclic triterpene, MA exhibits effective free radical scavenging capabilities. The purpose of this study was to explore the effects of MA on porcine early-stage embryonic development, oxidation resistance and mitochondrial function. Our results showed that 1 μM was the optimal concentration of MA, which resulted in dramatically increased blastocyst formation rates and improvement of blastocyst quality of in vitro-derived embryos from parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT). Further analysis indicated that MA supplementation not only significantly decreased the abundance of intracellular reactive oxygen species (ROS) and dramatically increased the abundance of intracellular reductive glutathione (GSH) in porcine early-stage embryos, but also clearly attenuated mitochondrial dysfunction and inhibited apoptosis. Moreover, Western blotting showed that MA supplementation upregulated OCT4 (p p p in vitro production (IVP) efficiency of porcine early-stage embryos.
