Jichu yixue yu linchuang (Jan 2020)
Comparison of biological characteristics of CD146 positive subpopulations of mesenchymal stem cells derived from three different sources
Abstract
Objective To characterize the biological features of CD146+ cells from umbilical cord mesenchymal stem cells(UCMSCs), adipose-derived mesenchymal stem cells(AMSCs) and bone marrow mesenchymal stem cells(BM-MSCs). Methods MSCs from three tissues were sorted by magnetically activated cell sorting(MACS) to obtain highly purified CD146+ subpopulations. The phenotypes and ultra-structures of these three MSC were analyzed by flow-cytometry and transmission electron microscopy. The differentiation abilities were tested by adipogenic and osteogenic inductions. The expression of genes relative to adipogenesis, osteogenesis, stemness or angiogenesis were determined by RT-qPCR. Results CD146+ cells derived from three kinds of tissues had similar ultra-structures and the ability of angiogenesis. CD146+ AMSCs expressed highest levels of the OCT-4, SOX2 and NANOG genes.Under the same induction conditions, CD146+ AMSCs showed higher potential for adipogenesis and CD146+BM-MSCs showed higher potential for osteogenesis.However, CD146+ UMSCs had little ability for adipogenesis and osteogenesis. All of CD146+ MSCs from 3 tissues expressed BFGF, VEGF, Ang-1 and EGF although CD146+ BM-MSCs had stronger ability of angiogenesis in vitro. Conclusions CD146+ MSCs derived from the 3 sources exhibit different biological characteristics.These results provide a useful biological basis for further studies relative to application of MSCs.
