Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 *4/*17 variant which confers intermediate drug metabolizing activity

Martina Bohndorf; Audrey Ncube; Lucas-Sebastian Spitzhorn; Jürgen Enczmann; Wasco Wruck; James Adjaye

doi:10.1016/j.scr.2017.10.004

Stem Cell Research (Dec 2017)

Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 4/17 variant which confers intermediate drug metabolizing activity

Martina Bohndorf,
Audrey Ncube,
Lucas-Sebastian Spitzhorn,
Jürgen Enczmann,
Wasco Wruck,
James Adjaye

Affiliations

Martina Bohndorf: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany
Audrey Ncube: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany
Lucas-Sebastian Spitzhorn: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany
Jürgen Enczmann: Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany
Wasco Wruck: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany
James Adjaye: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany

DOI: https://doi.org/10.1016/j.scr.2017.10.004
Journal volume & issue: Vol. 25, no. C
pp. 18 – 21

Abstract

Read online

SIX2-positive renal cells isolated from urine from a 51 year old male of African origin bearing the CYP2D6 *4/*17 variant were reprogrammed by nucleofection of a combination of two episomal-based plasmids omitting pathway (TGFβ, MEK and GSK3β) inhibition. The induced pluripotent stem cells (iPSCs) were characterized by immunocytochemistry, embryoid body formation, DNA-fingerprinting and karyotype analysis. Comparative transcriptome analyses with human embryonic stem cell lines H1 and H9 revealed a Pearson correlation of 0.9243 and 0.9619 respectively.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal