Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke
Elena A. Tukhovskaya,
Alina M. Ismailova,
Elvira R. Shaykhutdinova,
Gulsara A. Slashcheva,
Igor A. Prudchenko,
Inessa I. Mikhaleva,
Oksana N. Khokhlova,
Arkady N. Murashev,
Vadim T. Ivanov
Affiliations
Elena A. Tukhovskaya
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Alina M. Ismailova
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Elvira R. Shaykhutdinova
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Gulsara A. Slashcheva
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Igor A. Prudchenko
Laboratory of Peptide Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, 117997 Moscow, Russia
Inessa I. Mikhaleva
Laboratory of Peptide Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, 117997 Moscow, Russia
Oksana N. Khokhlova
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Arkady N. Murashev
Biological Testing Laboratory, Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Prospekt Nauki, 6, 142290 Moscow, Russia
Vadim T. Ivanov
Laboratory of Peptide Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, 117997 Moscow, Russia
Background and Objectives: Mutual effect of the preliminary and therapeutic intranasal treatment of SD rats with DSIP (8 days) on the outcome of focal stroke, induced with intraluminal middle cerebral occlusion (MCAO), was investigated. Materials and Methods: The groups were the following: MCAO + vehicle, MCAO + DSIP, and SHAM-operated. DSIP or vehicle was applied nasally 60 (±15) minutes prior to the occlusion and for 7 days after reperfusion at dose 120 µg/kg. The battery of behavioral tests was performed on 1, 3, 7, 14, and 21 days after MCAO. Motor coordination and balance and bilateral asymmetry were tested. At the end of the study, animals were euthanized, and their brains were perfused, serial cryoslices were made, and infarction volume in them was calculated. Results: Although brain infarction in DSIP-treated animals was smaller than in vehicle-treated animals, the difference was not significant. However, motor performance in the rotarod test significantly recovered in DSIP-treated animals. Conclusions: Intranasal administration of DSIP in the course of 8 days leads to accelerated recovery of motor functions.
