Numerical investigation of moving gel wall formation in a Y-shaped microchannel

Abstract

Abstract Molecular diffusive membranes play crucial roles in the field of microfluidics for biological applications e.g., 3D cell culture and biosensors. Hydrogels provide a range of benefits such as free diffusion of small molecules, cost-effectiveness, and the ability to be produced in bulk. Among various hydrogels, Pluronic F127 can be used for cell culture purposes due to its biocompatibility and flexible characteristics regarding its environment. Aqueous solutions of Pluronic F127 shows a reversible thermo-thickening property, which can be manipulated by introduction of ions. As a result, controlled diffusion of ions into the solution of Pluronic F127 can result in a controlled gel formation. In this study, the flow of immiscible solutions of Pluronic and sodium phosphate inside a Y-shaped microchannel is simulated using the level set method, and the effects of volume flow rates and temperature on the gel formation are investigated. It is indicated that the gel wall thickness can decrease by either increasing the Pluronic volume flow rate or increasing both volume flow rates while increasing the saline volume flow rate enhances the gel wall thickness. Below a critical temperature value, no gel wall is formed, and above that, a gel wall is constructed, with a thickness that increases with temperature. This setup can be used for drug screening, where gel wall provides an environment for drug-cell interactions. Article Highlights Parallel flow of Pluronic F127 and saline solutions inside a Y-shaped microchannel results in formation of a gel wall at their interface. The numerical analysis reveals the impact of each inlet flow rate and temperature on gel wall thickness and movement. The findings indicate that the gel wall has a low but steady velocity toward the saline solution. Graphical abstract

Keywords

• Pluronic F127
• Microchannel
• Hydrogel
• Gel wall
• Cell culture
• Level set method