Nature Communications (Mar 2018)

Benchmarking common quantification strategies for large-scale phosphoproteomics

  • Alexander Hogrebe,
  • Louise von Stechow,
  • Dorte B. Bekker-Jensen,
  • Brian T. Weinert,
  • Christian D. Kelstrup,
  • Jesper V. Olsen

DOI
https://doi.org/10.1038/s41467-018-03309-6
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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Quantitative phosphoproteomics has become a standard method in molecular and cell biology. Here, the authors compare performance and parameters of phosphoproteome quantification by LFQ, SILAC, and MS2-/MS3-based TMT and introduce a TMT-adapted algorithm for calculating phosphorylation site stoichiometry.