Fabrication of microgroove poly(lactic-co-glycolic acid) nerve guide conduit using dry-jet wet spinning for rat laryngeal recurrent nerve regeneration

Xiaohui Yang; Xixia Liu; Fangshen Xu; Shunxian Ji; Yuchao Sun; Zhaojun Song; Jie Song; Yulian Wu; Jun Yin

Materials & Design (Nov 2022)

Fabrication of microgroove poly(lactic-co-glycolic acid) nerve guide conduit using dry-jet wet spinning for rat laryngeal recurrent nerve regeneration

Xiaohui Yang,
Xixia Liu,
Fangshen Xu,
Shunxian Ji,
Yuchao Sun,
Zhaojun Song,
Jie Song,
Yulian Wu,
Jun Yin

Affiliations

Xiaohui Yang: Department of General Surgery, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Xixia Liu: The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China; Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China; School of Mechanical Engineering, Guizhou University, Guiyang 550025, China
Fangshen Xu: Department of General Surgery, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Shunxian Ji: The Pathology Department, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Yuchao Sun: The Central Lab, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Zhaojun Song: The Central Lab, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Jie Song: The Electromyography Department, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China
Yulian Wu: Department of General Surgery, The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China; Cancer Center, Zhejiang University, Hangzhou, Zhejiang, 310058, China; Corresponding authors at: The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China（YL.Wu); The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China (J. Yin).
Jun Yin: The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China; Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China; Corresponding authors at: The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu 322000, China（YL.Wu); The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China (J. Yin).

Journal volume & issue: Vol. 223
p. 111151

Abstract

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Recurrent laryngeal nerve (RLN) injury is a serious complication of thyroidectomy. Here, the degradable poly(lactic-co-glycolic acid) (PLGA) nerve guide conduits (NGCs) with microgroove or smooth inner surface were fabricated using a phase inversion-based dry-jet wet spinning method for RLN regeneration. The morphology and mechanical properties of NGCs were observed by scanning electron microscope (SEM) and a universal testing machine, showing that the porous NGC has good mechanical properties and is suitable for implantation. Further, in vitro PC12 cells cultured on the NGCs showed that microgroove conduit (GNGC) exhibited better cell adhesion, proliferation, and cell orientation. Both smooth and microgroove conduit (SNGC and GNGC) were transplanted into Sprague-Dawley (SD) rat models for assessing the repair effect on the 5-mm RLN gap. The results showed that GNGC was superior to SNGC in nerve regeneration and had similar effects to the autograft group in reducing muscle atrophy and restoring target organ function. Therefore, the degradable NGCs combined with groove structure could guide the regeneration of injured RLN, promote the partial functional recovery of target organs and prevent secondary surgical injury. This study contributes to the design and fabrication of nerve guide conduits and the application of tissue engineering in the field of RLN injury repair.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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