Amphiregulin is Not Essential for Induction of Contact Hypersensitivity

Akiko Yagami; Naoki Kajiwara; Keisuke Oboki; Tatsukuni Ohno; Hideaki Morita; Susan W Sunnarborg; Ko Okumura; Hideoki Ogawa; Hirohisa Saito; Susumu Nakae

Allergology International (Jan 2010)

Amphiregulin is Not Essential for Induction of Contact Hypersensitivity

Akiko Yagami,
Naoki Kajiwara,
Keisuke Oboki,
Tatsukuni Ohno,
Hideaki Morita,
Susan W Sunnarborg,
Ko Okumura,
Hideoki Ogawa,
Hirohisa Saito,
Susumu Nakae

Affiliations

Akiko Yagami: Department of Allergy and Immunology, National Research Institute for Child Health and Development,
Naoki Kajiwara: Department of Allergy and Immunology, National Research Institute for Child Health and Development,; Atopy Research Center, Juntendo University,
Keisuke Oboki: Department of Allergy and Immunology, National Research Institute for Child Health and Development,
Tatsukuni Ohno: Department of Allergy and Immunology, National Research Institute for Child Health and Development,
Hideaki Morita: Department of Allergy and Immunology, National Research Institute for Child Health and Development,; Department of Pediatrics, Keio University, School of Medicine,
Susan W Sunnarborg: Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, NC, USA.
Ko Okumura: Atopy Research Center, Juntendo University,
Hideoki Ogawa: Atopy Research Center, Juntendo University,
Hirohisa Saito: Department of Allergy and Immunology, National Research Institute for Child Health and Development,; Atopy Research Center, Juntendo University,
Susumu Nakae: Department of Allergy and Immunology, National Research Institute for Child Health and Development,; Atopy Research Center, Juntendo University,; Frontier Research Initiative, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Correspondence: Susumu Nakae, PhD, Frontier Research Initiative, Institute of Medical Science, University of Tokyo, 4−6−1 Shirokanedai, Minato, Tokyo 108−8639, Japan.

Journal volume & issue: Vol. 59, no. 3
pp. 277 – 284

Abstract

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ABSTRACT: Background: Amphiregulin (AR) is expressed in Th2 cells, rather than Th1 cells, and plays an important role in Th2 cell/cytokine-mediated host defense against nematodes. We also found earlier that AR mRNA expression was strongly upregulated in inflamed tissue during Th2 cell/cytokine-mediated fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS), suggesting a contribution of AR to the induction of those responses. Methods: To elucidate the role of AR in the induction of FITC- or dinitrofluorobenzene (DNFB)-induced CHS, AR-deficient mice were sensitized and/or challenged with FITC or DNFB epicutaneously. The levels of FITC- mediated skin dendritic cell (DC) migration and FITC-specific lymph node cell proliferation and cytokine production were assessed by flow cytometry, [3H]-thymidine incorporation and ELISA, respectively, after FITC sensitization. The degree of ear swelling, the activities of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) in inflammatory sites and the levels of FITC-specific immunoglobulin (Ig) in sera were determined by histological analysis, colorimetric assay and ELISA, respectively, after FITC challenge. Results: DC migration and FITC-specific lymph node cell proliferation and cytokine production were normal in the AR-deficient mice. Ear swelling, tissue MPO and EPO activities and FITC-specific serum Ig levels were also similar in AR-deficient and -sufficient mice. Conclusions: Amphiregulin is not essential for the induction of FITC- or DNFB-induced CHS responses in mice. KEY WORDS: amphiregulin, contact dermatitis, EGF, mast cells, Th2 cell/cytokine

Published in Allergology International

ISSN: 1323-8930 (Print); 1440-1592 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.journals.elsevier.com/allergology-international/

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