Biocompatible 3D Matrix with Antimicrobial Properties
Alberto Ion,
Ecaterina Andronescu,
Dragoș Rădulescu,
Marius Rădulescu,
Florin Iordache,
Bogdan Ștefan Vasile,
Adrian Vasile Surdu,
Madalina Georgiana Albu,
Horia Maniu,
Mariana Carmen Chifiriuc,
Alexandru Mihai Grumezescu,
Alina Maria Holban
Affiliations
Alberto Ion
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Ecaterina Andronescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Dragoș Rădulescu
Department of Orthopedics and Traumatology, Bucharest University Hospital, 169 Splaiul Independentei, 050098 Bucharest, Romania
Marius Rădulescu
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Florin Iordache
Department of Fetal and Adult Stem Cell Therapy, Institute of Cellular Biology and Pathology of Romanian Academy, 8, B.P. Hasdeu, 050568 Bucharest, Romania
Bogdan Ștefan Vasile
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Adrian Vasile Surdu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Madalina Georgiana Albu
Department of Collagen, Branch of Leather and Footwear Research, National Institute of Research and Development for Textile and Leather, 93 I. Minulescu Street, 031215 Bucharest, Romania
Horia Maniu
Department of Fetal and Adult Stem Cell Therapy, Institute of Cellular Biology and Pathology of Romanian Academy, 8, B.P. Hasdeu, 050568 Bucharest, Romania
Mariana Carmen Chifiriuc
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Alina Maria Holban
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), β-cyclodextrin (β-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering.
