STAR Protocols (Dec 2023)

In vivo functional brain mapping using ultra-high-field fMRI in awake common marmosets

  • Alessandro Zanini,
  • Audrey Dureux,
  • Azadeh Jafari,
  • Kyle M. Gilbert,
  • Peter Zeman,
  • Miranda Bellyou,
  • Alex Li,
  • Cheryl Vander Tuin,
  • Stefan Everling

Journal volume & issue
Vol. 4, no. 4
p. 102586

Abstract

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Summary: The common marmoset (Callithrix jacchus) is gaining attention in the field of cognitive neuroscience. The development of an effective protocol for fMRI data acquisition in awake marmosets is a key factor in developing reliable comparative studies. Here, we describe a protocol to obtain fMRI data in awake marmosets using auditory and visual stimulation. We describe steps for surgical and anesthesia procedures, MRI training, and positioning the marmosets within an MRI-compatible body restraint. We then detail fMRI scanning and preprocessing of functional images.For complete details on the use and execution of this protocol, please refer to Jafari et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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