Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats

Sandra Pereira; Margaret K. Hahn; Bailey Humber; Talha Chaudhry; Sally Wu; Sri Mahavir Agarwal; Nanka Dimitrova; Adria Giacca

STAR Protocols (Sep 2024)

Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats

Sandra Pereira,
Margaret K. Hahn,
Bailey Humber,
Talha Chaudhry,
Sally Wu,
Sri Mahavir Agarwal,
Nanka Dimitrova,
Adria Giacca

Affiliations

Sandra Pereira: Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Physiology, University of Toronto, Toronto, ON, Canada; Corresponding author
Margaret K. Hahn: Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada; Department of Pharmacology, University of Toronto, Toronto, ON, Canada
Bailey Humber: Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada
Talha Chaudhry: Department of Physiology, University of Toronto, Toronto, ON, Canada
Sally Wu: Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada
Sri Mahavir Agarwal: Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada
Nanka Dimitrova: Department of Comparative Medicine, University of Toronto, Toronto, ON, Canada
Adria Giacca: Department of Physiology, University of Toronto, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada; Corresponding author

Journal volume & issue: Vol. 5, no. 3
p. 103143

Abstract

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Summary: In rats, cannulation of the jugular vein and the carotid artery precedes the use of the hyperinsulinemic euglycemic clamp to determine insulin sensitivity in vivo. Here, we present a vascular surgery protocol to allow the infusion of substances via the vein and the collection of blood samples from the artery on the day of the hyperinsulinemic euglycemic clamp. We describe steps for preparing for and performing catheterization surgery. We then detail procedures for clamp preparation and its use.For complete details on the use and execution of this protocol, please refer to Pereira et al.1,2,3 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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