MicroRNA Profile in Peripheral Blood Mononuclear Cells from Hepatitis B Virus Infected Patients

Abstract

Introduction and aim. The pathogenesis of hepatitis B virus (HBV)-related liver diseases remains not fully understood. Here, we aim to explore the potential roles of dysregulated miRNAs in chronic hepatitis B (CHB) and HBV-related acute-on-chronic liver failure (ACLF).Material and methods. MiRNA microarray was conducted in peripheral blood mononuclear cells (PBMCs) obtained from healthy donors or patients with CHB or ACLF. Altered expression of miRNAs was further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Finally, the differentially expressed miRNAs and their target genes were subjected to bioinformatics analysis.Results. The miRNA microarray identified 45 up-regulated and 62 down-regulated miRNAs with a fold change > 1.5. Expression of eight miRNAs was validated using qRT-PCR analysis, which was consistent with miRNA microarray analysis. Bioinformatics analysis indicated that multiple biological processes and signaling pathways were affected by these miRNAs and a miRNA-gene regulatory network was generated with Cytoscape.Conclusion. The current study provided a global view of miRNA expression in PBMCs from CHB and ACLF patients. Functional analysis showed that multiple biological processes and signaling pathways were modulated by these miRNAs. These data provide intriguing insights into the molecular pathogenesis of HBV-related liver diseases, which deserve further investigation.

Keywords

• Non-coding RNA
• Chronic hepatitis B
• Bioinformatics