Pharmaceutics (Jun 2023)

Development of a Gene and Nucleic Acid Delivery System for Skeletal Muscle Administration via Limb Perfusion Using Nanobubbles and Ultrasound

  • Shohko Sekine,
  • Sayaka Mayama,
  • Nobuaki Nishijima,
  • Takuo Kojima,
  • Yoko Endo-Takahashi,
  • Yuko Ishii,
  • Hitomi Shiono,
  • Saki Akiyama,
  • Akane Sakurai,
  • Sanae Sashida,
  • Nobuhito Hamano,
  • Rui Tada,
  • Ryo Suzuki,
  • Kazuo Maruyama,
  • Yoichi Negishi

DOI
https://doi.org/10.3390/pharmaceutics15061665
Journal volume & issue
Vol. 15, no. 6
p. 1665

Abstract

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Strategies for gene and nucleic acid delivery to skeletal muscles have been extensively explored to treat Duchenne muscular dystrophy (DMD) and other neuromuscular diseases. Of these, effective intravascular delivery of naked plasmid DNA (pDNA) and nucleic acids into muscles is an attractive approach, given the high capillary density in close contact with myofibers. We developed lipid-based nanobubbles (NBs) using polyethylene-glycol-modified liposomes and an echo-contrast gas and found that these NBs could improve tissue permeability by ultrasound (US)-induced cavitation. Herein, we delivered naked pDNA or antisense phosphorodiamidate morpholino oligomers (PMOs) into the regional hindlimb muscle via limb perfusion using NBs and US exposure. pDNA encoding the luciferase gene was injected with NBs via limb perfusion into normal mice with application of US. High luciferase activity was achieved in a wide area of the limb muscle. DMD model mice were administered PMOs, designed to skip the mutated exon 23 of the dystrophin gene, with NBs via intravenous limb perfusion, followed by US exposure. The number of dystrophin-positive fibers increased in the muscles of mdx mice. Combining NBs and US exposure, which can be widely delivered to the hind limb muscles via the limb vein, could be an effective therapeutic approach for DMD and other neuromuscular disorders.

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