Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
Sei-ichi Kawahara,
Chisato Ishihara,
Kiriko Matsumoto,
Shogo Senga,
Koichiro Kawaguchi,
Ayaka Yamamoto,
Jutalak Suwannachot,
Yasunori Hamauzu,
Hidefumi Makabe,
Hiroshi Fujii
Affiliations
Sei-ichi Kawahara
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Chisato Ishihara
Graduate School of Science and Technology, Department of Biomedical Engineering, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Kiriko Matsumoto
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Shogo Senga
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Koichiro Kawaguchi
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Ayaka Yamamoto
Graduate School of Science and Technology, Department of Biomedical Engineering, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Jutalak Suwannachot
Graduate School of Science and Technology, Department of Agriculture, Division of Food Science and Biotechnology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Yasunori Hamauzu
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan; Graduate School of Science and Technology, Department of Agriculture, Division of Food Science and Biotechnology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan
Hidefumi Makabe
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan; Graduate School of Science and Technology, Department of Agriculture, Division of Food Science and Biotechnology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan; Department of Biomolecular Innovation, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan; Corresponding author.
Hiroshi Fujii
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan; Graduate School of Science and Technology, Department of Biomedical Engineering, Shinshu University, 8304 Minami-minowa Kami-ina, Nagano, 399-4598, Japan; Department of Biomolecular Innovation, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Minami-minowa, Kami-ina, Nagano, 399-4598, Japan; Corresponding author.
The aim of the present study was to characterize and evaluate the anti-cancer activity of proanthocyanidin-enriched fractions from adzuki beans. For this purpose, we concentrated proanthocyanidins from adzuki beans (Vigna angularis) into five fractions using Amberlite XAD-1180N, Toyopearl HW40F, and Sepacore C-18 reverse-phase flash column chromatography. Proanthocyanidin-enriched fractions were characterized as (epi)catechin hexamer, heptamer, and octamer, epigallocatechin-(epi)catechin pentamer, and epigallocatechin-(epi)catechin hexamer using electrospray ionization time-of-flight mass spectrometry and thiolytic degradation. These fractions showed significant anti-cancer activity against the human PC-3 prostate cancer cell line. They also significantly suppressed the expression of the fatty acid-binding protein 5 gene, which plays critical roles in cell growth and metastasis in prostate cancer.
