Green synthesised CuNPs using Alhagi maurorum extract and its ability to amelioration of Mycoplasma pneumoniae infected pneumonia mice model

Abstract

In this study, copper nanoparticles were synthesised according to green chemistry rules using the aqueous extract of Alhagi maurorum. The green-synthesised CuNPs were characterised using different techniques such as EDX, FE-SEM, XRD and FT-IR. The FE-SEM results confirm spherical morphology for the nanoparticles with a size of 42.68–68.87 nm. In vivo, the Mycoplasma pneumonia injection was applied for inducing pneumonia in the BALB/c mice, also the treatment was with CuNPs. The serum parameters such as inflammatory mediators (IL-6, TNF-α, IL-8, TGF and IL-1), total cell counts, and total protein content levels were evaluated. CuNPs regulated the levels of the inflammatory mediators in the infected mice. The cellular arrangements at the histopathological images were ameliorated with the administration of CuNPs. At the antioxidant test, the CuNPs and BHT removed 50% of free radicals at the concentrations of 181 and 110 µg/ml. In conclusion, the results revealed the ameliorative property of CuNPs green-formulated by A. maurorum aqueous extract on M. pneumoniae infected pneumonia mice model.

Keywords

• Alhagi maurorum
• copper nanoparticles
• Mycoplasma pneumonia
• mouse model
• inflammatory cytokines