Enhanced bone regeneration capability of chitosan sponge coated with TiO2 nanoparticles

Radyum Ikono; Ni Li; Nanda Hendra Pratama; Agnia Vibriani; Diah Retno Yuniarni; Muhammad Luthfansyah; Boy Muchlis Bachtiar; Endang Winiati Bachtiar; Kamarza Mulia; Mohammad Nasikin; Hideaki Kagami; Xianqi Li; Etik Mardliyati; Nurul Taufiqu Rochman; Tokiko Nagamura-Inoue; Arinobu Tojo

Biotechnology Reports (Dec 2019)

Enhanced bone regeneration capability of chitosan sponge coated with TiO2 nanoparticles

Radyum Ikono,
Ni Li,
Nanda Hendra Pratama,
Agnia Vibriani,
Diah Retno Yuniarni,
Muhammad Luthfansyah,
Boy Muchlis Bachtiar,
Endang Winiati Bachtiar,
Kamarza Mulia,
Mohammad Nasikin,
Hideaki Kagami,
Xianqi Li,
Etik Mardliyati,
Nurul Taufiqu Rochman,
Tokiko Nagamura-Inoue,
Arinobu Tojo

Affiliations

Radyum Ikono: Division of Bionanotechnology, Nano Center Indonesia, Jl. Raya Serpong, 15310, Tangerang Selatan, Indonesia; Department of Metallurgical Engineering, Sumbawa University of Technology, Jl. Raya Olat Maras, 84371, Nusa Tenggara Barat, Indonesia; Division of Molecular Therapy, Institute of Medical Science, The University of Tokyo, 7 Chome-3-1 Hongo, 113-8654, Tokyo, Japan; Corresponding author at: Division of Bionanotechnology, Nano Center Indonesia, Jl. Raya Serpong, 15310, Tangerang Selatan, Indonesia.
Ni Li: Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, 1780 Hirookagobara, Shiojiri, Nagano-Prefecture, 399-0704, Japan
Nanda Hendra Pratama: Division of Bionanotechnology, Nano Center Indonesia, Jl. Raya Serpong, 15310, Tangerang Selatan, Indonesia
Agnia Vibriani: Department of Biology, Bandung Institute of Technology, Jl. Ganesha No. 10, 40132, Bandung, Indonesia
Diah Retno Yuniarni: Department of Chemistry, University of Indonesia, Jl. Margonda Raya, 16424, Depok, Indonesia
Muhammad Luthfansyah: Division of Bionanotechnology, Nano Center Indonesia, Jl. Raya Serpong, 15310, Tangerang Selatan, Indonesia
Boy Muchlis Bachtiar: Oral Science Laboratory, Department of Dentistry, University of Indonesia, Jl. Salemba Raya, 10430, Central Jakarta, Indonesia
Endang Winiati Bachtiar: Oral Science Laboratory, Department of Dentistry, University of Indonesia, Jl. Salemba Raya, 10430, Central Jakarta, Indonesia
Kamarza Mulia: Department of Chemical Engineering, University of Indonesia, Jl. Margonda Raya, 16424, Depok, Indonesia
Mohammad Nasikin: Department of Chemical Engineering, University of Indonesia, Jl. Margonda Raya, 16424, Depok, Indonesia
Hideaki Kagami: Division of Molecular Therapy, Institute of Medical Science, The University of Tokyo, 7 Chome-3-1 Hongo, 113-8654, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, 1780 Hirookagobara, Shiojiri, Nagano-Prefecture, 399-0704, Japan; Department of General Medicine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, 7 Chome-3-1 Hongo, 113-8654, Tokyo, Japan
Xianqi Li: Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, 1780 Hirookagobara, Shiojiri, Nagano-Prefecture, 399-0704, Japan
Etik Mardliyati: Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology (BPPT), PUSPIPTEK Area, 15314, Tangerang Selatan, Indonesia
Nurul Taufiqu Rochman: Research Center for Physics, Indonesian Institute of Science (LIPI), PUSPIPTEK Area, 15314, Tangerang Selatan, Indonesia
Tokiko Nagamura-Inoue: Department of Cell Processing and Transfusion, The Institute of Medical Science, The University of Tokyo, 7 Chome-3-1 Hongo, 113-8654, Tokyo, Japan
Arinobu Tojo: Division of Molecular Therapy, Institute of Medical Science, The University of Tokyo, 7 Chome-3-1 Hongo, 113-8654, Tokyo, Japan

Journal volume & issue: Vol. 24

Abstract

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Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO2 nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO2 nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO2 nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO4− band observed in FTIR results. qPCR analysis supported chitosan - TiO2 sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO2 treated group. Finally, cytotoxicity analysis supported the fact that TiO2 nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO2 nanoparticles sponges could be a potential novel scaffold for bone tissue engineering. Keywords: Bone regeneration, Chitosan, Sponges, TiO2 nanoparticles, Tissue engineering

Published in Biotechnology Reports

ISSN: 2215-017X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biotechnology-reports/

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