A Nanozymatic-Mediated Smartphone Colorimetric Sensing Platform for the Detection of Dimethyl Phthalate (DMP) and Dibutyl Phthalate (DBP)
Wenhui Li,
Xuecheng Zhang,
Haojie Zhang,
Cheng Zhang,
Yingjie Chen,
Cong Li,
Yonghong Hu,
Xiaoping Yu,
Biao Zhang,
Xiaodong Lin
Affiliations
Wenhui Li
College of Food and Light Industry, Nanjing Tech University, Nanjing 211816, China
Xuecheng Zhang
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Haojie Zhang
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Cheng Zhang
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Yingjie Chen
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Cong Li
Agriculture and Rural Bureau of Zhuozhou, Zhuozhou 072750, China
Yonghong Hu
College of Food and Light Industry, Nanjing Tech University, Nanjing 211816, China
Xiaoping Yu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Biao Zhang
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Xiaodong Lin
Zhuhai UM Science & Technology Research Institute, Zhuhai 519000, China
Plasticizers are a type of toxic substance that may remain in food, posing significant health risks including carcinogenic, teratogenic, mutagenic, and other adverse effects. In this study, a novel strategy was employed by combining Pt@Au nanozymes with high catalytic properties to created two catalytic signal probes, designated as Pt@Au@Ab1 and Pt@Au@Ab2, specifically designed for the detection of dimethyl phthalate (DMP) and dibutyl phthalate (DBP). These catalytic signal probes served as the foundation for the development of a colorimetric immunoassay, enabling the simultaneous detection of both DMP and DBP. The colorimetric immunoassay is capable of detecting DMP in the range of 0.5–100 μg/L with a limit of detection as low as 0.1 μg/L and DBP in the range of 1–32 μg/L with a low limit of detection of 0.5 μg/L. The developed immunoassay can be used for the determination of the DMP and DBP in baijiu and plastic bottled drinks. The recovery rate is in the range of 96.4% and 100.5% and the coefficient of variation is between 1.0% and 7.2%. This innovative colorimetric immunoassay offers a robust tool for the simultaneous quantification of DMP and DBP in real samples.
