Typing class I HLA-A gene using a nested PCR-RFLP procedure

Abstract

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.

Keywords

• Human leukocyte antigen-A
• HLA-A
• Typing
• RFLP
• Polymorphism
• Major histocompatibility complex