PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

Gabrielle J. Grundy; Luis M. Polo; Zhihong Zeng; Stuart L. Rulten; Nicolas C. Hoch; Pathompong Paomephan; Yingqi Xu; Steve M. Sweet; Alan W. Thorne; Antony W. Oliver; Steve J. Matthews; Laurence H. Pearl; Keith W. Caldecott

doi:10.1038/ncomms12404

Nature Communications (Aug 2016)

PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

Gabrielle J. Grundy,
Luis M. Polo,
Zhihong Zeng,
Stuart L. Rulten,
Nicolas C. Hoch,
Pathompong Paomephan,
Yingqi Xu,
Steve M. Sweet,
Alan W. Thorne,
Antony W. Oliver,
Steve J. Matthews,
Laurence H. Pearl,
Keith W. Caldecott

Affiliations

Gabrielle J. Grundy: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Luis M. Polo: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Zhihong Zeng: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Stuart L. Rulten: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Nicolas C. Hoch: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Pathompong Paomephan: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Yingqi Xu: Department of Life Sciences, Cross-faculty NMR centre, Faculty of Natural Sciences, Imperial College London
Steve M. Sweet: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Alan W. Thorne: Institute of Biomedical and Biomolecular Sciences, University of Portsmouth
Antony W. Oliver: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Steve J. Matthews: Department of Life Sciences, Cross-faculty NMR centre, Faculty of Natural Sciences, Imperial College London
Laurence H. Pearl: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Keith W. Caldecott: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex

DOI: https://doi.org/10.1038/ncomms12404
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

Read online

Chromosomal single-strand DNA breaks occur frequently and require repair to avoid disease outcomes. Here, the authors show that in bird cells, PARP3 accelerates this repair, and use structural biology and cell biology techniques to reveal details of the mechanism of action.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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