Scientific Reports (Mar 2022)

Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic α-helical peptides

  • Yuki Takechi-Haraya,
  • Takashi Ohgita,
  • Mana Kotani,
  • Hiroki Kono,
  • Chihiro Saito,
  • Hiroko Tamagaki-Asahina,
  • Kazuchika Nishitsuji,
  • Kenji Uchimura,
  • Takeshi Sato,
  • Ryuji Kawano,
  • Kumiko Sakai-Kato,
  • Ken-ichi Izutsu,
  • Hiroyuki Saito

DOI
https://doi.org/10.1038/s41598-022-08876-9
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 11

Abstract

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Abstract We previously developed an amphipathic arginine-rich peptide, A2-17, which has high ability to directly penetrate across cell membranes. To understand the mechanism of the efficient cell-penetrating ability of the A2-17 peptide, we designed three structural isomers of A2-17 having different values of the hydrophobic moment and compared their membrane interaction and direct cell penetration. Confocal fluorescence microscopy revealed that cell penetration efficiency of peptides tends to increase with their hydrophobic moment, in which A2-17 L14R/R15L, an A2-17 isomer with the highest hydrophobic moment, predominantly remains on plasma cell membranes. Consistently, Trp fluorescence analysis indicated the deepest insertion of A2-17 L14R/R15L into lipid membranes among all A2-17 isomers. Electrophysiological analysis showed that the duration and charge flux of peptide-induced pores in lipid membranes were prominent for A2-17 L14R/R15L, indicating the formation of stable membrane pores. Indeed, the A2-17 L14R/R15L peptide exhibited the strongest membrane damage to CHO-K1 cells. Atomic force microscopy quantitatively defined the peptide-induced membrane perturbation as the decrease in the stiffness of lipid vesicles, which was correlated with the hydrophobic moment of all A2-17 isomers. These results indicate that optimal membrane perturbation by amphipathic A2-17 peptide is critical for its efficient penetration into cells without inducing stabilized membrane pores.