BioTechniques (Jan 2017)

AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms

  • Sergio Callejas,
  • Rebeca Álvarez,
  • Alberto Benguria,
  • Ana Dopazo

DOI
https://doi.org/10.2144/000114504
Journal volume & issue
Vol. 62, no. 1
p. xvi

Abstract

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Protocol Summary We have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.