Simplified fluorometric method for the determination of plasma glycerol

Mayer B. Davidson; Robert Karjala

Journal of Lipid Research (Nov 1970)

Simplified fluorometric method for the determination of plasma glycerol

Mayer B. Davidson,
Robert Karjala

Affiliations

Mayer B. Davidson: Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024
Robert Karjala: Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024

Journal volume & issue: Vol. 11, no. 6
pp. 609 – 612

Abstract

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A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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Abstract

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