Bacteria Community Vertical Distribution and Its Response Characteristics to Waste Degradation Degree in a Closed Landfill

Abstract

The diversity, community structure and vertical distribution characteristics of bacteria in the surface and subsurface soil and water samples of a closed landfill in Shanghai Jiading District were investigated to reveal the relationships between natural waste degradation degree and the succession of bacterial community composition. High-throughput sequencing of bacteria 16S rDNA genes was used to analyze the bacterial community structure and diversity. The results showed that the diversity of bacteria in the surface samples was higher than that in the deep samples. Proteobacteria was the dominant phylum in all the samples, and the percentage increased with depth. At the genus level, Thiobacillus, Pseudomonas, Aquabacterium, and Hydrogenophaga were the dominant genera in surface, medium, deep and ultra-deep soils, respectively. The Bray–Curtis dissimilarity of the soil bacterial communities in the same layer was small, indicating that the community composition of the samples in the same layer was similar. The RDA result showed that ammonium, nitrate, pH and C/N significantly influenced the community structure of soil bacteria. This is of great relevance to understand the effect of natural waste degradation on bacterial communities in closed landfills.

Keywords

• bacteria
• vertical distribution
• waste degradation
• ammonium
• closed landfill