Sodium New Houttuyfonate Affects Transcriptome and Virulence Factors of Pseudomonas aeruginosa Controlled by Quorum Sensing

Yeye Zhao; Longfei Mei; Yuanqing Si; Jiadi Wu; Jing Shao; Jing Shao; Tianming Wang; Guiming Yan; Changzhong Wang; Changzhong Wang; Daqiang Wu; Daqiang Wu; Daqiang Wu

doi:10.3389/fphar.2020.572375

Frontiers in Pharmacology (Oct 2020)

Sodium New Houttuyfonate Affects Transcriptome and Virulence Factors of Pseudomonas aeruginosa Controlled by Quorum Sensing

Yeye Zhao,
Longfei Mei,
Yuanqing Si,
Jiadi Wu,
Jing Shao,
Jing Shao,
Tianming Wang,
Guiming Yan,
Changzhong Wang,
Changzhong Wang,
Daqiang Wu,
Daqiang Wu,
Daqiang Wu

Affiliations

Yeye Zhao: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Longfei Mei: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Yuanqing Si: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Jiadi Wu: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Jing Shao: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Jing Shao: Key Laboratory of Chinese Herbal Compound Formula in Anhui Province, Anhui University of Chinese Medicine, Hefei, China
Tianming Wang: Key Laboratory of Chinese Herbal Compound Formula in Anhui Province, Anhui University of Chinese Medicine, Hefei, China
Guiming Yan: Division of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, China
Changzhong Wang: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Changzhong Wang: Key Laboratory of Chinese Herbal Compound Formula in Anhui Province, Anhui University of Chinese Medicine, Hefei, China
Daqiang Wu: Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China
Daqiang Wu: Key Laboratory of Chinese Herbal Compound Formula in Anhui Province, Anhui University of Chinese Medicine, Hefei, China
Daqiang Wu: Division of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, China

DOI: https://doi.org/10.3389/fphar.2020.572375
Journal volume & issue: Vol. 11

Abstract

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As a major opportunistic pathogen, Pseudomonas aeruginosa can produce various virulence factors and form biofilms. These processes are controlled by the quorum sensing (QS) system. Sodium new houttuyfonate (SNH) is an adduct of houttuyfonate, the main component of the common Chinese medicine plant Houttuynia cordata, which has antibacterial and anti-inflammatory effects. We evaluated the effect of SNH on P. aeruginosa biofilms, virulence factors, and transcription. Transcriptome analysis showed that the key rhlI and pqsA genes of the P. aeruginosa QS system were down-regulated after SNH treatment. SNH reduces proteases and pyocyanin production and inhibits biofilm formation by regulating the P. aeruginosa QS system. SNH also changes the expression of genes related to virulence factors and biofilms (lasA, lasB, lecA, phzM, pqsA, and pilG). These results suggested that the mechanism of SNH against P. aeruginosa by affecting the expression of biofilm and virulence factors controlled by quorum sensing.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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