Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

Tarun S. Nambiar; Pierre Billon; Giacomo Diedenhofen; Samuel B. Hayward; Angelo Taglialatela; Kunheng Cai; Jen-Wei Huang; Giuseppe Leuzzi; Raquel Cuella-Martin; Andrew Palacios; Anuj Gupta; Dieter Egli; Alberto Ciccia

doi:10.1038/s41467-019-11105-z

Nature Communications (Jul 2019)

Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

Tarun S. Nambiar,
Pierre Billon,
Giacomo Diedenhofen,
Samuel B. Hayward,
Angelo Taglialatela,
Kunheng Cai,
Jen-Wei Huang,
Giuseppe Leuzzi,
Raquel Cuella-Martin,
Andrew Palacios,
Anuj Gupta,
Dieter Egli,
Alberto Ciccia

Affiliations

Tarun S. Nambiar: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Pierre Billon: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Giacomo Diedenhofen: Naomi Berrie Diabetes Center and Department of Pediatrics, Columbia University Irving Medical Center
Samuel B. Hayward: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Angelo Taglialatela: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Kunheng Cai: Naomi Berrie Diabetes Center and Department of Pediatrics, Columbia University Irving Medical Center
Jen-Wei Huang: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Giuseppe Leuzzi: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Raquel Cuella-Martin: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Andrew Palacios: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Anuj Gupta: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center
Dieter Egli: Naomi Berrie Diabetes Center and Department of Pediatrics, Columbia University Irving Medical Center
Alberto Ciccia: Department of Genetics and Development, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

DOI: https://doi.org/10.1038/s41467-019-11105-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Manipulating DNA repair pathways can be used to improve the outcomes of CRISPR-based genome editing. Here the authors derive an enhanced RAD18 variant that suppresses 53BP1 recruitment to DNA double-strand breaks to enhance homology-mediated repair.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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