Comparison of the Quality of Protein Crystals Grown by CLPC Seeds Method
Jin Li,
Er-Kai Yan,
Yue Liu,
Zi-Qing Wu,
Ya-Li Liu,
Hai Hou,
Chen-Yan Zhang,
Qin-Qin Lu,
Xu-Dong Deng,
Da-Chuan Yin
Affiliations
Jin Li
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Er-Kai Yan
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Yue Liu
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Zi-Qing Wu
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Ya-Li Liu
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Hai Hou
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Chen-Yan Zhang
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Qin-Qin Lu
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Xu-Dong Deng
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
Da-Chuan Yin
Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shanxi, China
We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.
