A peptide dataset for target analysis of human complement system proteins
Thais Cristina Tirado,
Kelly Cavalcanti Machado,
Patrícia Shigunov,
Michel Batista,
Fabiano Borges Figueiredo
Affiliations
Thais Cristina Tirado
Laboratório de Biologia Celular, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil; Laboratório de Referência em Leishmanioses, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil; Corresponding authors.
Kelly Cavalcanti Machado
Plataforma de Espectrometria de Massas RPT02H, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil
Patrícia Shigunov
Laboratório de Biologia Básica de Células-Tronco, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil
Michel Batista
Plataforma de Espectrometria de Massas RPT02H, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil; Corresponding authors.
Fabiano Borges Figueiredo
Laboratório de Biologia Celular, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil; Laboratório de Referência em Leishmanioses, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil
The targeted LC-MS/MS method has been widely applied for peptide quantification, offering sensibility, specificity, and reproducibility to the analysis. However, it requires the prior selection of targets, including the construction of a spectral library. Here, we present a dataset comprising peptide mass spectra for targeted LC-MS/MS method setup, applied to a set of human complement system proteins. Additionally, we selected a group of peptides and demonstrated their stability and reproducibility in quantification. This dataset is invaluable for studies aiming at the quantification of the complement system proteins by targeted LC-MS/MS, as it provides data for spectral library construction and a list of selected peptides.
