International Journal of Nanomedicine (Dec 2024)
Regenerative Potential Nanomedicine of Adipocyte Stem Cell-Derived Exosomes in Senescent Skin Tissue
Abstract
An-Na Li,1,* Jing-Hua Sun,1,2,* Syafiqah Saidin,3,4 Jee Syuen Cheah,4,5 Chia-Hung Kuo,6 Ling Li,1 Jia-Shen Li,1 Ru-Yu Bai,1 Yong Diao,1 Hui-Min David Wang5,7– 9 1School of Medicine, Huaqiao University, Quanzhou, Fujian, 362021, People’s Republic of China; 2Hebei Key Laboratory of Basic Medicine for Diabetes, Shijiazhuang Second Hospital, Shijiazhuang, Hebei, 050000, People’s Republic of China; 3IJN-UTM Cardiovascular Engineering Centre, Institute of Human Centered Engineering, Universiti Teknologi Malaysia, Johor Bahru, Johor, 81310, Malaysia; 4Department of Biomedical Engineering & Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Johor Bahru, Johor, 81310, Malaysia; 5Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China; 6Department of Fishery and Food Science, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, Republic of China; 7Regenerative Medicine and Cell Therapy Research Center; and Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China; 8Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, Republic of China; 9Center of Applied Nanomedicine, National Cheng Kung University, Tainan, Taiwan, Republic of China*These authors contributed equally to this workCorrespondence: Hui-Min David Wang, Graduate Institute of Biomedical Engineering, National Chung Hsing University, No. 145, Xingda Road, South District, Taichung City, 402, Taiwan, Republic of China, Tel +886 4 22840733 #651 ; +886 935753718, Email [email protected] Yong Diao, School of Medicine, Huaqiao University, Quanzhou, Fujian, 362021, People’s Republic of China, Email [email protected]: Skin is the first-line barrier defense against infection, irradiation, and toxins, but is prone to natural aging (intrinsic aging) and environmental factors (extrinsic aging). Hence, there is an increasing urgency to explore an effective treatment for aging skin. This study was focused on testing the potential of utilizing adipocyte stem cell derived exosomal as nanomedicine to regenerate the dermal layer and counteract signs of skin aging.Methods: The harvested stem cells from adipose tissues were isolated, cultured, and then starved. The centrifugation of cell cultures medium yielded the human adipose-derived stem cells conditional medium (HADSCs-CM). Collagen secretion and fibroblast viability of human fibroblasts (Hs68) were measured in the presence of HADSCs-CM. The dermal layer, vascular endothelial growth factor (VEGF), and collagen levels were evaluated on the mice animal models between the treatments with and without HADSCs-CM.Results: Western blotting, transmission electron microscopy (TEM), and dynamic light scattering (DLS) confirmed that the functional particles in HADSCs-CM were exosomes. When Hs68 fibroblasts were treated with HADSCs-CM, both cell viability and collagen secretion increased in a dose-dependent manner. Following the post-ultraviolet A (post-UVA) exposure, the mice exposed to the HADSCs-CM have decreased dermal thickness and VEGF expression and increased collagen volume compared to the non-HADSCs-CM exposed mice (control group).Conclusion: HADSCs-CM significantly alleviated signs of skin senescence, including reduced dermal thickness, decreased VEGF expression, and enhanced collagen production. Exosomes, identified in the HADSCs-CM, are the functional component of these regenerative effects. This study highlights that the exosomal nanomedicine found in HADSCs-CM could regenerate skin, boost collagen production, improve fibroblast cell viability, and contain functional exosomes. Keywords: adipocyte stem cells, collagen, dermal regeneration, exosomal nanomedicine, skin aging
