Toxin Profiles of Okadaic Acid Analogues and Other Lipophilic Toxins in <i>Dinophysis</i> from Japanese Coastal Waters
Hajime Uchida,
Ryuichi Watanabe,
Ryoji Matsushima,
Hiroshi Oikawa,
Satoshi Nagai,
Takashi Kamiyama,
Katsuhisa Baba,
Akira Miyazono,
Yuki Kosaka,
Shinnosuke Kaga,
Yukihiko Matsuyama,
Toshiyuki Suzuki
Affiliations
Hajime Uchida
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
Ryuichi Watanabe
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
Ryoji Matsushima
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
Hiroshi Oikawa
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
Satoshi Nagai
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
Takashi Kamiyama
National Research Institute of Fisheries and Environment of Inland Sea, Japan Fisheries Research and Education Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Katsuhisa Baba
Central Fisheries Research Institute, Fisheries Research Department, Hokkaido Research Organization, 238, Hamanakacho, Yoichi-cho, Yoichi-gun, Hokkaido 046-8555, Japan
Akira Miyazono
Kushiro Fisheries Research Institute, Fisheries Research Department, Hokkaido Research Organization, 4-25, Nakahamacho, Kushiro-city, Hokkaido 085-0027, Japan
Yuki Kosaka
Aomori Prefectural Industrial Technology Research Center, Fisheries Research Institute, Hiranai, Higashitsugarugun, Aomori 039-3381, Japan
Shinnosuke Kaga
Iwate Fisheries Technology Center, 3-75-3 Hirata, Kamaishi, Iwate 026-0001, Japan
Yukihiko Matsuyama
Seikai National Fisheries Research Institute, Japan Fisheries Research and Education Agency, 1551-8, Taira-machi, Nagasaki-shi, Nagasaki 851-2213, Japan
Toshiyuki Suzuki
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan
The identification and quantification of okadaic acid (OA)/dinophysistoxin (DTX) analogues and pectenotoxins (PTXs) in Dinophysis samples collected from coastal locations around Japan were evaluated by liquid chromatography mass spectrometry. The species identified and analyzed included Dinophysis fortii, D. acuminata, D. mitra (Phalacroma mitra), D. norvegica, D. infundibulus, D. tripos, D. caudata, D. rotundata (Phalacroma rotundatum), and D. rudgei. The dominant toxin found in D. acuminata was PTX2 although some samples contained DTX1 as a minor toxin. D. acuminata specimens isolated from the southwestern regions (Takada and Hiroshima) showed characteristic toxin profiles, with only OA detected in samples collected from Takada. In contrast, both OA and DTX1, in addition to a larger proportion of PTX2, were detected in D. acuminata from Hiroshima. D. fortii showed a toxin profile dominated by PTX2 although this species had higher levels of DTX1 than D. acuminata. OA was detected as a minor toxin in some D. fortii samples collected from Yakumo, Noheji, and Hakata. PTX2 was also the dominant toxin found among other Dinophysis species analyzed, such as D. norvegica, D. tripos, and D. caudata, although some pooled picked cells of these species contained trace levels of OA or DTX1. The results obtained in this study re-confirm that cellular toxin content and profiles are different even among strains of the same species.
