Drug Design, Development and Therapy (Jun 2024)
The Mechanism of Catalpol to Improve Oxidative Damage of Dermal Fibroblasts Based on Nrf2/HO-1 Signaling Pathway
Abstract
Xiaona Lang,1 Liyan Xu,2 Lu Li,1 Xin Feng1 1Pharmacy Department, Tianjin Hospital, Tianjin, People’s Republic of China; 2Orthopedic Department, Tianjin Hospital, Tianjin, People’s Republic of ChinaCorrespondence: Xin Feng, Pharmacy Department, Tianjin Hospital, Tianjin, People’s Republic of China, Tel +86-13652001152, Email [email protected]: Catalpol, as a natural medicine small-molecule drug, has been proven to have anti-inflammatory and antioxidant pharmacological effects.Methods: The effect of catalpol on oxidative damage of mouse epidermal fibroblast L929 model and its mechanism were investigated by using hydrogen peroxide model, CCK8 method, flow cytometry, and Western blot.Results: The effect of catalpol on Nrf2/HO-1 signaling pathway was further studied to improve oxidative stress in cell models. The results showed that catalpol had no cytotoxicity to L929 cells, and inhibited the apoptosis of L929 cells after oxidative damage in a concentration-dependent manner, thus playing a role in cell protection. The oxidative damage of cells was inhibited by up-regulating the expression of the signature protein of Nrf2/HO-1 signaling pathway and inhibiting the interstitial formation of cells.Conclusion: This study is a preliminary study on the protective function of catalpol against oxidation and apoptosis in dermal fibroblasts, which can provide a theoretical basis and drug guidance for promoting skin wound healing in the later stage.Keywords: catalpol, oxidative damage, Nrf2/HO-1, dermal fibroblasts, oxidative stress
