Molecules (Jun 2024)

Increasing 1,4-Diaminobutane Production in <i>Escherichia coli</i> by Optimization of Cofactor PLP and NADPH Synthesis

  • Tong Sun,
  • Yongcan Zhao,
  • Jinjin Wang,
  • Wenke Kang,
  • Xiangxiang Sun,
  • Yanling Sun,
  • Meixue Chu,
  • Zhengyu Liu,
  • Fuping Lu,
  • Ming Li

DOI
https://doi.org/10.3390/molecules29133094
Journal volume & issue
Vol. 29, no. 13
p. 3094

Abstract

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1,4-diaminobutane is widely used in the industrial production of polymers, pharmaceuticals, agrochemicals and surfactants. Owing to economic and environmental concerns, there has been a growing interest in using microbes to produce 1,4-diaminobutane. However, there is lack of research on the influence of cofactors pyridoxal phosphate (PLP) and NADPH on the synthesis of 1,4-diaminobutane. PLP serves as a cofactor of ornithine decarboxylase in the synthesis of 1,4-diaminobutane. Additionally, the synthesis of 1 mol 1,4-diaminobutane requires 2 mol NADPH, thus necessitating consideration of NADPH balance in the efficient synthesis of 1,4-diaminobutane by Escherichia coli. The aim of this study was to enhance the synthesis efficiency of 1,4-diaminobutane through increasing production of PLP and NADPH. By optimizing the expression of the genes associated with synthesis of PLP and NADPH in E. coli, cellular PLP and NADPH levels increased, and the yield of 1,4-diaminobutane also increased accordingly. Ultimately, using glucose as the primary carbon source, the yield of 1,4-diaminobutane in the recombinant strain NAP19 reached 272 mg/L·DCW, by increased 79% compared with its chassis strain.

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