Tracking Root Interactions System (TRIS) Experiment and Quality Control
Hassan Massalha,
Elisa Korenblum,
Orr Shapiro,
Aharoni Asaph
Affiliations
Hassan Massalha
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Elisa Korenblum
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Orr Shapiro
Department of Food Sciences, Institute for Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7528809, Israel
Aharoni Asaph
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.
