Détection de souches multi-résistantes d’Escherichia coli d’origine aviaire dans la région de Rabat-Salé-Zemmour-Zaer

Abstract

Antimicrobial resistance surveillance is a standard measure in the sanitary policies. In order to monitor multi-resistance within APEC strains, a study was conducted on 370 broiler chickens farms from the Rabat-Salé-Zemmour-Zaer region in Morocco. The isolation of Escherichia coli was made from lesions of avian colibacillosis. Biochemical identification, serotyping and antimicrobial susceptibility tests as well as phenotypic identifications on lactamases resistance were carried out on the isolates. Serogrouping showed that 66.7 % of the isolates belong to the dominant serotypes O78, O 2 and O1. Drug sensitivity tests showed extremely high levels of resistance to oxytetracyclin (100 %), amoxicillin (90.9%), trimethoprim + sulphamethoxazol (82.2 %), enrofloxacin (75.9 %) and florfenicol (61.5 %). Low frequencies of resistances were noted for gentamicin (24.8 %), fosfomycin (16.1 %) and colistin (2%). In parallel, tests for the determination of the phenotype of resistance to beta-lactamases and cephalosporinases on 250 strains revealed that 66.1% of the strains were classified as class A penicillinases; 14.8% were class C cephalosporinases. The ESBL class A was found in 11.1% of the tested stains and only 3.7% were classified as wild-type strains. The multi-resistance is very high since 97.4% and 55.1% of the strains were respectively resistant to three and five antibacterial drugs.

Keywords

• Antibio-resitance
• antibiotics
• Escherichia coli
• colibacillosis
• poultry
• Morocco