Microbial population changes and metabolic shift of candidatus accumulibacter under low temperature and limiting polyphosphate

Abstract

This study explored the microbial population dynamics of Accumulibacter (Acc) at low temperature and metabolic shift to limiting polyphosphate (Poly-P) in enhanced biological phosphorus removal (EBPR) system. The Accumulibacter-enriched EBPR systems, fed with acetate (HAc) and propionate (HPr) at 10 ± 1 °C respectively, were operated for 60 days in two identical SBR reactors (SBR-1 and SBR-2). The phosphorus removal performance in two systems was stable at 10 ± 1 °C, while the microbial community structure changed. Compared with the population structure in seed sludge, Accumulibacter clades reduced in the HAc system, while Acc I increased significantly in the HPr system. Low temperature was beneficial to the formation of granular sludge in the EBPR system, and the sludge granulation in the HAc system was more homogeneous than that in the HPr system. Accumulibacter in the HPr system can get ATP through glycogen accumulating metabolism (GAM) under limiting Poly-P condition at 10 ± 1 °C, while that in the HAc system cannot. This work suggests that poly-P levels can affect the metabolic pathway of Accumulibacter in EBPR systems under low temperature. HIGHLIGHTS Low temperature was more beneficial to the proliferation of Acc I feeding with HPr.; Sludge granulation was achieved in the EBPR system at 10 ± 1 °C.; Acc in HPr system can behave as GAM under the limiting Poly-P at 10 ± 1 °C.;

Keywords

• candidatus accumulibacter phosphatis
• granular sludge
• limiting polyphosphate
• low temperature
• metabolic shift