Frontiers in Bioengineering and Biotechnology (May 2023)

Dynamic and self-biodegradable polysaccharide hydrogel stores embryonic stem cell construct under ambient condition

  • Kuan Yang,
  • Wei Wei,
  • Li Ting Gao,
  • Xin Yi Zhao,
  • Zhenqi Liu,
  • Jianhui Li,
  • Haopeng Li,
  • Hideyuki Miyatake,
  • Yoshihiro Ito,
  • Yong Mei Chen

DOI
https://doi.org/10.3389/fbioe.2023.1169124
Journal volume & issue
Vol. 11

Abstract

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The proper microenvironment is critical for the storage and transportation of embryonic stem cells (ESCs). To mimic a dynamic 3D microenvironment as it exists in vivo and consider “off-the-shelf” availability reaching the destination, we proposed an alternative approach that allows for facile storage and transportation of stem cells in the form of ESCs-dynamic hydrogel construct (CDHC) under ambient conditions. To form CDHC, mouse embryonic stem cells (mESCs) were in-situ encapsulated within a polysaccharide-based dynamic and self-biodegradable hydrogel. After storing CDHC in a sterile and hermetic environment for 3 days and then transferring to a sealed vessel with fresh medium for another 3 days, the large and compact colonies retained a 90% survival rate and pluripotency. Furthermore, after transporting and arriving at the destination, the encapsulated stem cell could be automatically released from the self-biodegradable hydrogel. After continuous cultivation of 15 generations of retrieved cells, automatically released from the CDHC, the mESCs underwent 3D encapsulation, storage, transportation, release, and continuous long-term subculture; resumed colony forming capacity and pluripotency were revealed by stem cell markers both in protein and mRNA levels. We believe that the dynamic and self-biodegradable hydrogel provides a simple, cost-effective, and valuable tool for storing and transporting “ready-to-use” CDHC under ambient conditions, facilitating “off-the-shelf” availability and widespread applications.

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