The Lancet Regional Health - Southeast Asia (Aug 2023)

Normative range of various serum hormonal parameters among Indian women of reproductive age: ICMR-PCOS task force study outcomeResearch in context

  • Mohd Ashraf Ganie,
  • Subhankar Chowdhury,
  • Vanita Suri,
  • Beena Joshi,
  • Prasanta Kumar Bhattacharya,
  • Sarita Agrawal,
  • Neena Malhotra,
  • Rakesh Sahay,
  • Puthiyaveettil Khadar Jabbar,
  • Abilash Nair,
  • Roya Rozati,
  • Amlin Shukla,
  • Rabiya Rashid,
  • Idrees A. Shah,
  • Haroon Rashid,
  • Imtiyaz Ahmad Wani,
  • Taruna Arora,
  • Bharati Kulkarni,
  • Mohd Ashraf Ganie,
  • Bharti Kulkarni,
  • Amlin Shukla,
  • Taruna Arora,
  • Subhankar Chowdhury,
  • Vanita Suri,
  • Beena Joshi,
  • Prasanta Kumar Bhattacharya,
  • Sarita Agrawal,
  • Neena Malhotra,
  • Rakesh Sahay,
  • Puthiyaveettil Khadar Jabbar,
  • Roya Rozati,
  • Imtiyaz Ahmad Wani,
  • V. Sreenivas,
  • Mukesh Srivastava,
  • Abilash Nair,
  • Parvaiz Koul,
  • Zafar Amin Shah,
  • Shariq Masoodi,
  • Tabassum Parvez,
  • Ghulam Nabi Yatoo,
  • Naseer Ahmed,
  • Tariq Gojwari,
  • Muzafar Wani,
  • Seema Qayoom,
  • Wahid Khan,
  • Rama Walia,
  • Dipankar De,
  • Aashima Arora,
  • Tulika Singh,
  • Neena Khanna,
  • Nitish Naik,
  • Shyam Prakash,
  • Nandita Gupta,
  • Rajesh Sagar,
  • Jai Bhagwan Sharma,
  • Devasenathipathy Kandasamy,
  • Narendra Kuber Bodhey,
  • Eli Mohapatra,
  • Sabah Siddiqui,
  • Gourisankar Kamilya,
  • Archana Singh,
  • Pradip Mukhopadhyay,
  • Mousumi Mukhopadhyay,
  • Nehar Ranjan Sarkar,
  • Alice Abraham Ruram,
  • Jessy Abraham,
  • Saroj Kumar Pati,
  • Dibakar Sahu,
  • Lokesh Kumar Singh,
  • Donboklang Lynser,
  • Manika Agrawal,
  • Ashok Anand,
  • Shahina Begum,
  • Anushree Patil,
  • Shrabani Mukherjee,
  • Hemangini Thakkar,
  • Aruna Ramaiah,
  • Lakshman Rao,
  • Sudha Bindu,
  • Malathi Ponnuru,
  • Nadeem Ahmad,
  • Sujatha Rani,
  • Aleem Ahmed Khan,
  • Sultan Rizwan Ahmed,
  • Prabhakar Rao,
  • C. Nirmala,
  • Anuja Elizabeth George,
  • Jayasree Leelamma,
  • Jayakumari,
  • Gaurav Thakur,
  • Saba Noor,
  • Shaikh Sharmeen,
  • Gaivee Vinam Meshram,
  • Shouvik Choudhury Aafia Rashid,
  • Wasia Showkat,
  • Rohina Bashir,
  • Rabiya Rashid,
  • Pieu Adhikary,
  • Subhasish Pramanik,
  • Rahul Harish,
  • Mudasir Makhdoomi,
  • Mudasir Fayaz,
  • Nafeez Rehman,
  • Neha Ravi,
  • Ajay Kumar,
  • Siffali Chandrakar,
  • Nithlesh Kumar,
  • Sudipta Banerjee,
  • Gazala Hasan,
  • Nitin Deshpande,
  • Himali Vadhan,
  • Rita Parab,
  • Humaira Minhaj,
  • Balaji Bhasker,
  • Shaik Iqbal Ahmed,
  • Ram Babu,
  • Arya Suresh

Journal volume & issue
Vol. 15
p. 100226

Abstract

Read online

Summary: Background: The hormonal profile varies considerably with age, gender, ethnicity, diet or physiological state of an individual. Limited population-specific studies have studied the variations in hormonal parameters among apparently healthy women. We aimed to analyse the biological reference interval for various hormonal parameters in the reproductive-aged healthy Indian women. Methods: Out of 3877 participants that were clinically evaluated, 1441 subjects were subjected to laboratory investigations. All participants underwent a detailed clinical, biochemical and hormonal profiling. The hormone analysis was carried out at a single centre using a uniform methodology. Among the participants evaluated for biochemical and hormonal parameters, subjects that presented any abnormal profile or had incomplete investigations (n = 593) were excluded for further analysis. Findings: The mean age (±SD) of the subjects retained in the final analysis (n = 848) was 29.9 (±6.3) years. In the present study, the biological reference interval (2.5th–97.5th centile) observed were: serum T4: μg/dL (5.23–12.31), TSH: μg/mL (0.52–4.16) and serum prolactin: ng/mL (5.13–37.35), LH: mIU/mL (2.75–20.68), FSH: mIU/mL 2.59–15.12), serum total testosterone: ng/mL (0.06–0.68), fasting insulin: mIU/mL (1.92–39.72), morning cortisol: μg/dL (4.71–19.64), DHEAS:μg/dL (50.61–342.6) and SHBG: nmol/L (21.37–117.54). Unlike T4, TSH, LH, and E2, the biological reference interval for prolactin, FSH, testosterone, C-peptide insulin and DHEAS varied when the subjects were stratified by age (p < 0.05). The comparative analysis showed marginal differences in the normative ranges for the hormones analysed among different populations. Interpretation: Our first large composite data on hormonal measures will benefit future endeavours to define biological reference intervals in reproductive-aged Indian women. Funding: The study was financially supported by the grant-in-aid from ICMR vide file No:5/7/13337/2015-RBMH.

Keywords