Colorimetric plasmon resonance microfluidics on nanohole array sensors

Austin Hsiao; Manas Ranjan Gartia; Te-Wei Chang; Xinhao Wang; Pakapreud Khumwan; Gang Logan Liu

Sensing and Bio-Sensing Research (Sep 2015)

Colorimetric plasmon resonance microfluidics on nanohole array sensors

Austin Hsiao,
Manas Ranjan Gartia,
Te-Wei Chang,
Xinhao Wang,
Pakapreud Khumwan,
Gang Logan Liu

Affiliations

Austin Hsiao: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Manas Ranjan Gartia: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA; Corresponding authors at: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, and Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Te-Wei Chang: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Xinhao Wang: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Pakapreud Khumwan: Department of Bioengineering, University of Washington, Seattle, WA 98195, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Gang Logan Liu: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Corresponding authors at: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, and Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.

Journal volume & issue: Vol. 5
pp. 24 – 32

Abstract

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We present the label-free colorimetric visualization in microfluidics using plasmon resonance on a large-area and over a wide field-of-view (>100 mm2) nanohole array device called nanoLycurgus Cup Array (nanoLCA). We demonstrate the spectral detection and colorimetric sensing of static solutions of different concentrations of glycerol–water confined in parallel microfluidic channels integrated with nanoLCA. Taking advantage of the large sensor area and the colorimetric sensing capability of nanoLCA, we visualize in real-time the modulation of two immiscible solutions (water and oil), generated with integrated flow-focus microfluidics, in a label-free manner. Finite Element Method (FEM) based simulation tool (COMSOL) was used to verify the droplet formation in the microfluidics. Finite Difference Time Domain (FDTD) electromagnetic simulation was used to identify the resonance modes of the plasmonic sensor. Finally, we demonstrate the real-time monitoring of streptavidin–biotin biomolecular interaction with the plasmonic biosensor. Keywords: Microfluidics, Surface plasmon, Colorimetric sensor, Nanohole array, Protein binding, Droplet formation

Published in Sensing and Bio-Sensing Research

ISSN: 2214-1804 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Engineering (General). Civil engineering (General)
Website: http://www.journals.elsevier.com/sensing-and-bio-sensing-research/

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