Prediction of Methotrexate toxicity in Algerian Rheumatoid Arthritis Patients: Implication of GGH rs11545078 polymorphism

Abstract

Objectives - The aim of this study is to determine the impact of the gamma glutamyl hydrolase (GGH) c.452C>T (dbSNP Id: rs 11545078) polymorphism on the Methotrexate (MTX) adverse drug reactions (ADRs) and on MTX gastro intestinal toxicity (GIT) in rheumatoid arthritis (RA) patients. Materials and methods - Sixty-one patients with RA are enrolled in this study. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Data were analyzed by χ2. Results - Our findings suggest that the frequencies distributions of alleles/genotypes of this polymorphism were similar in both groups with and without ADRs. However, there is a significant distribution of GGH 452TT (p=0.03) and GGH 452CT+452TT (p=0.02) genotypes between the groups with and without GIT. The same correlation was found for GGH 452T allele (p=0.008). Conclusion - We have shown for the first time in the West Algerian population that the GGH c.452C>T polymorphism influences the MTX gastro-intestinal toxicity.

Keywords

• GGH
• Methotrexate
• Rheumatoid arthritis
• Algeria
• polymorphism