STAR Protocols (Sep 2021)

Single-cell lactate production rate as a measure of glycolysis in endothelial cells

  • Devin Harrison,
  • David Wu,
  • Jun Huang,
  • Yun Fang

Journal volume & issue
Vol. 2, no. 3
p. 100807

Abstract

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Summary: Heterogeneous metabolism supports critical single-cell functions. Here, we describe deep-learning-enabled image analyses of a genetically encoded lactate-sensing probe which can accurately quantify metabolite levels and glycolytic rates at the single-cell level. Multiple strategies and test data have been included to obviate possible obstacles including successful sensor expression and accurate segmentation. This protocol reliably discriminates between metabolically diverse subpopulations which can be used to directly link metabolism to functional phenotypes by integrating spatiotemporal information, genetic or pharmacological perturbations, and real-time metabolic states.For complete details on the use and execution of this protocol, please refer to Wu et al. (2021a).

Keywords